This technique employs a separation method based on the specific precipitation of low density lipoproteins (LDL) by polyvinyl sulphate in serum, sedimentation of the precipitate by centrifugation and subsequent assay as residual cholesterol of the remaining lipoproteins (HDL + VLDL) contained in the clear supernatant. LDL-cholesterol is calculated by difference, subtracting the supernatant cholesterol from the total cholesterol in the sample.
